|
Sign In to gain access to subscriptions and/or personal tools.
|
Inflammatory markers and IL-6 polymorphism in peripheral arterial disease with and without diabetes mellitus
P Danielsson
Department of Vascular Diseases, University Hospital MAS, Malmö, Sweden, Peter.Danielsson{at}skane.seanddanod@telia.com
L Truedsson
Department of Clinical Microbiology and Immunology, University Hospital, Lund, Sweden
K-F Eriksson
Department of Vascular Diseases, University Hospital MAS, Malmö, Sweden
L Norgren
Department of Vascular Diseases, University Hospital MAS, Malmö, Sweden
Peripheral arterial disease (PAD) is a manifestation of systemic atherosclerosis, recognized as an inflammatory disease of the vessel wall, probably accelerated by diabetes mellitus (DM). Elevated interleukin (IL)-6 levels have been associated with increased cardiovascular morbidity and a common polymorphism has been identified in the promoter region of the IL-6 gene. The aim of this prospective study was to investigate inflammatory mediators in PAD patients (±DM) and to investigate a possible relationship to the IL-6 gene polymorphism. Five groups of patients (DM, intermittent claudication ±DM, critical limb ischemia (CLI) ±DM) and a control group of 20 individuals each were included. Hemoglobin, high sensitive C-reactive protein (hsCRP), creatinine, blood lipids, white blood cells (WBC); CD11b/CD18; vascular cell adhesion molecule (sVCAM-1), intercellular adhesion molecule (sICAM-1), sE-selectin, sP-selectin; IL-6, IL-8, tumour necrosis factor (TNF) , sTNF-R1 and sTNF-R2 were analysed. The IL-6 gene polymorphism was determined in all groups and also compared with 200 healthy controls from a larger study of blood donors. In a multiple regression analysis, adjusted for gender, smoking and age, the effect of CLI was significantly (p < 0.05) associated with elevated levels of the WBC count, hsCRP, proinflammatory cytokines (IL-6, TNF-R1-2) and endothelial (sICAM, sVCAM) and WBC (CD11b gran) markers. The effect of less advanced PAD (intermittent claudication) was related to an increased concentration of sVCAM-1 and the number of monocytes and granulocytes. DM or leg ulcers were not significantly related to any of the markers. No significant difference in frequency of the various IL-6 genotypes was found between the groups or when compared with the group of 200 blood donors (p > 0.3). Activation of cytokines, endothelial cells and WBC was related to the Fontaine stage of PAD but not to the presence of DM or ulcers. No association was found between the polymorphism in the IL-6 promoter region and PAD.
Key Words: diabetes mellitus • inflammation • interleukin-6 • peripheral arterial disease • polymorphism
Vascular Medicine, Vol. 10, No. 3,
191-198 (2005)
DOI: 10.1191/1358863x05vm617oa
 CiteULike  Complore  Connotea  Del.icio.us  Digg  Reddit  Technorati  Twitter What's this?
This article has been cited by other articles:
|
|
|
|
 
E. Zintzaras and N. Zdoukopoulos
A Field Synopsis and Meta-Analysis of Genetic Association Studies in Peripheral Arterial Disease: The CUMAGAS-PAD Database
Am. J. Epidemiol.,
July 1, 2009;
170(1):
1 - 11.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Isma, J. Barani, I. Mattiasson, B. Lindblad, and A. Gottsater
Lipid-Lowering Therapy is Related to Inflammatory Markers and 3-Year Mortality in Patients With Critical Limb Ischemia
Angiology,
October 1, 2008;
59(5):
542 - 548.
[Abstract]
[PDF]
|
|
|
|
|
|
|
H.R.S. Girn, N.M. Orsi, and S. Homer-Vanniasinkam
An overview of cytokine interactions in atherosclerosis and implications for peripheral arterial disease
Vascular Medicine,
November 1, 2007;
12(4):
299 - 309.
[Abstract]
[PDF]
|
|
|
|
|
|
|
L. Qi, R. M. van Dam, J. B. Meigs, J. E. Manson, D. Hunter, and F. B. Hu
Genetic variation in IL6 gene and type 2 diabetes: tagging-SNP haplotype analysis in large-scale case-control study and meta-analysis
Hum. Mol. Genet.,
June 1, 2006;
15(11):
1914 - 1920.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
